What are the limitations of PCR (polymerase chain reaction) and do you overcome it?

Due to the extremely high sensitivity of PCR, contamination from non-template PCR present in the lab environment (from bacteria, viruses, and your own DNA) presents a real problem. Precautions therefore must be taken. Several steps can be taken to prevent and minimize PCR contamination including the use of aerosol barrier tip filters, laminar flow cabinet, and a special preparation area for PCR separate from the DNA isolation area.